<i>Pseudomonas syringae</i> pv. <i>phaseolicola</i> (P.s. phaseolicola) is one of about 45 acknowledged pathovars inside the <i>P. syringae</i> group and is the causal agent of halo-blight illness of beans. DNA from this bacterium digested to completion with two totally different restriction enzymes, <i>Pac</i>I and <i>Pme</i>I, yielded 15 and 16 fragments, respectively. These have been separated utilizing PFGE and sized by comparability to recognized <em>molecular</em> mass markers. The <i>P.s. phaseolicola</i> chromosome was decided to be roughly 5.64 Mb in dimension.
To hyperlink the totally different fragments obtained right into a round chromosome map for each enzymes, 150 random Tn<i>5</i> mutants of <i>P.s. phaseolicola</i> have been used as a supply of DNA and the identification of the band carrying the transposon ‘tag’ in every mutant was accomplished after PFGE and Southern hybridization of an entire chromosomal digestion utilizing a Tn<i>5</i> probe. Partial digestions of DNA from totally different Tn<i>5</i> mutants ‘tagging’ particular bands have been then generated and the full and partial merchandise of the digestion separated by PFGE and recognized with a Tn<i>5</i> probe.
By calculating the dimension of the partial merchandise, it was then attainable to hyperlink totally different bands right into a bodily map. This is the first report on the development of a bodily map of a member of the P. syringae group and needs to be invaluable for <em>molecular</em> <em>genetic</em> evaluation on this species and in evolutionary or taxonomic research when in comparison with comparable information obtained for any of the different acknowledged pathovars. In Caulobacter crescentus, this nanofilament, although essential for floor colonization, has by no means been completely investigated at the molecular degree.
Bacterial pili are proteinaceous motorized nanomachines that play numerous practical roles together with floor adherence, bacterial movement, and virulence. The surface-contact sensor kind IVc (or Tad) pilus is broadly distributed in each Gram-positive and Gram-negative micro organism. As Caulobacter assembles a number of floor appendages at particular phases of the cell cycle, we designed a fluorescence-based display screen to selectively research single piliated cells and mixed it with atomic pressure microscopy and genetic manipulation to quantify the nanoscale adhesion of the kind IVc pilus to hydrophobic substrates.
Deep studying approaches for pure product discovery from plant endophytic microbiomes
Plant microbiomes should not solely numerous, but in addition seem to host an enormous pool of secondary metabolites holding nice promise for bioactive pure merchandise and drug discovery. Yet, most microbes inside crops look like uncultivable, and for these that may be cultivated, their metabolic potential lies largely hidden by regulatory silencing of biosynthetic genes. The current explosion of highly effective interdisciplinary approaches, together with multi-omics strategies to handle multi-trophic interactions and synthetic intelligence-based computational approaches to deduce distribution of operate, collectively current a paradigm shift in high-throughput approaches to pure product discovery from plant-associated microbes.
Arguably, the key to characterizing and harnessing this biochemical capability is determined by a novel, systematic method to characterize the triggers that activate secondary metabolite biosynthesis by molecular or genetic indicators from the host plant, members of the wealthy ‘in planta’ group, or from the setting. This assessment explores breakthrough approaches for pure product discovery from plant microbiomes, emphasizing the promise of deep studying as a software for endophyte bioprospecting, endophyte biochemical novelty prediction, and endophyte regulatory management.
It concludes with a proposed pipeline to harness international databases (genomic, metabolomic, regulomic, and chemical) to uncover and unsilence fascinating pure merchandise. In gentle of this rising understanding, the G. tritici-wheat interplay might present a mannequin research system for root-infecting fungal pathogens of cereals.
Take-All Disease: New Insights into an Important Wheat Root Pathogen
Take-all illness, attributable to the fungal root pathogen Gaeumannomyces tritici, is taken into account to be the most vital root illness of wheat worldwide. Here we assessment the advances in take-all analysis over the final 15 years, specializing in the identification of new sources of genetic resistance in wheat family members and the function of the microbiome in illness growth. We additionally spotlight current breakthroughs in the molecular interactions between G. tritici and wheat, together with genome and transcriptome analyses. These new findings will support the growth of novel management methods in opposition to take-all illness.
The growing demand for environment friendly and strong processes in the purification of monoclonal antibodies (mAbs) has not too long ago introduced frontal chromatography to the forefront. Applied throughout the sprucing step, it permits the elimination of excessive molecular weight aggregates from the goal product, reaching excessive purities. Typically, this course of is operated in batch utilizing a single column, which makes it intrinsically subjected to a purity-yield tradeoff. This implies that excessive purities can solely be achieved at the value of decreasing the product yield and vice versa.
) Pfu DNA Polymerase (2X Pre-mix, ready to use) |
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| S122 | GeneOn | 5x100 rcs | EUR 302 |
) CA15-3 (Ready-To-Use) |
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| A00078-0002 | ScyTek Laboratories | 2 ml | EUR 84 |
CA15-3 (Ready-To-Use) |
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| A00078-0007 | ScyTek Laboratories | 7 ml | EUR 116 |
CA15-3 (Ready-To-Use) |
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| A00078-0025 | ScyTek Laboratories | 25 ml | EUR 245 |
) Melanoma; Pan (Ready-To-Use) |
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| A00134-0002 | ScyTek Laboratories | 2 ml | EUR 140 |
Melanoma; Pan (Ready-To-Use) |
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| A00134-0007 | ScyTek Laboratories | 7 ml | EUR 272 |
Melanoma; Pan (Ready-To-Use) |
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| A00134-0025 | ScyTek Laboratories | 25 ml | EUR 717 |
) Mirskys Fixative (ready-to-use) |
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| NAT1300 | National Diagnostics | 1USGALLON | EUR 80 |
Mirskys Fixative (ready-to-use) |
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| NAT1302 | National Diagnostics | EACH | EUR 190 |
 Eco-Stain, ready to use |
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| DT81413 | Bio Basic | 1ml | EUR 102.2 |
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) Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use) |
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| S113 | GeneOn | 2x100 rcs | EUR 66 |
Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use) |
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| S114 | GeneOn | 10x100 rcs | EUR 193 |
) Desmin, Clone D33 (Ready-To-Use) |
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| A00007-0002 | ScyTek Laboratories | 2 ml | EUR 93 |
Desmin, Clone D33 (Ready-To-Use) |
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| A00007-0007 | ScyTek Laboratories | 7 ml | EUR 140 |
Desmin, Clone D33 (Ready-To-Use) |
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| A00007-0025 | ScyTek Laboratories | 25 ml | EUR 361 |
) Melanoma; Clone HMB45 (Ready-To-Use) |
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| A00019-0002 | ScyTek Laboratories | 2 ml | EUR 183 |
Melanoma; Clone HMB45 (Ready-To-Use) |
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| A00019-0007 | ScyTek Laboratories | 7 ml | EUR 258 |
Melanoma; Clone HMB45 (Ready-To-Use) |
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| A00019-0025 | ScyTek Laboratories | 25 ml | EUR 781 |
) Neurofilament; Clone 2F11 (Ready-To-Use) |
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| A00020-0002 | ScyTek Laboratories | 2 ml | EUR 96 |
Neurofilament; Clone 2F11 (Ready-To-Use) |
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| A00020-0007 | ScyTek Laboratories | 7 ml | EUR 144 |
Neurofilament; Clone 2F11 (Ready-To-Use) |
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| A00020-0025 | ScyTek Laboratories | 25 ml | EUR 370 |
; Polyclonal (Ready-To-Use)) Lysozyme (Muramidase); Polyclonal (Ready-To-Use) |
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| A00033-0002 | ScyTek Laboratories | 2 ml | EUR 79 |
Lysozyme (Muramidase); Polyclonal (Ready-To-Use) |
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| A00033-0007 | ScyTek Laboratories | 7 ml | EUR 108 |
Lysozyme (Muramidase); Polyclonal (Ready-To-Use) |
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| A00033-0025 | ScyTek Laboratories | 25 ml | EUR 252 |
) S-100; Polyclonal (Ready-To-Use) |
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| A00036-0002 | ScyTek Laboratories | 2 ml | EUR 79 |
S-100; Polyclonal (Ready-To-Use) |
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| A00036-0007 | ScyTek Laboratories | 7 ml | EUR 108 |
S-100; Polyclonal (Ready-To-Use) |
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| A00036-0025 | ScyTek Laboratories | 25 ml | EUR 218 |
) CD74; Clone LN2 (Ready-To-Use) |
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| A00048-0002 | ScyTek Laboratories | 2 ml | EUR 84 |
CD74; Clone LN2 (Ready-To-Use) |
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| A00048-0007 | ScyTek Laboratories | 7 ml | EUR 116 |
CD74; Clone LN2 (Ready-To-Use) |
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| A00048-0025 | ScyTek Laboratories | 25 ml | EUR 245 |
) Thyroglobulin; Clone 2H11 (Ready-To-Use) |
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| A00108-0002 | ScyTek Laboratories | 2 ml | EUR 80 |
Thyroglobulin; Clone 2H11 (Ready-To-Use) |
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| A00108-0007 | ScyTek Laboratories | 7 ml | EUR 127 |
Thyroglobulin; Clone 2H11 (Ready-To-Use) |
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| A00108-0025 | ScyTek Laboratories | 25 ml | EUR 280 |
) CD31; Clone C31.7 (Ready-To-Use) |
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| A00110-0002 | ScyTek Laboratories | 2 ml | EUR 104 |
CD31; Clone C31.7 (Ready-To-Use) |
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| A00110-0007 | ScyTek Laboratories | 7 ml | EUR 183 |
CD31; Clone C31.7 (Ready-To-Use) |
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| A00110-0025 | ScyTek Laboratories | 25 ml | EUR 449 |
; Polyclonal (Ready-To-Use)) p40 (DeltaNp63); Polyclonal (Ready-To-Use) |
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| A00112-0002 | ScyTek Laboratories | 2 ml | EUR 116 |
p40 (DeltaNp63); Polyclonal (Ready-To-Use) |
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| A00112-0007 | ScyTek Laboratories | 7 ml | EUR 213 |
p40 (DeltaNp63); Polyclonal (Ready-To-Use) |
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| A00112-0025 | ScyTek Laboratories | 25 ml | EUR 537 |
) Kappa; Clone L1C1 (Ready-To-Use) |
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| A00113-0002 | ScyTek Laboratories | 2 ml | EUR 69 |
Kappa; Clone L1C1 (Ready-To-Use) |
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| A00113-0007 | ScyTek Laboratories | 7 ml | EUR 95 |
Kappa; Clone L1C1 (Ready-To-Use) |
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| A00113-0025 | ScyTek Laboratories | 25 ml | EUR 188 |
) CD56; Clone 123C3 (Ready-To-Use) |
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| A00121-0002 | ScyTek Laboratories | 2 ml | EUR 104 |
CD56; Clone 123C3 (Ready-To-Use) |
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| A00121-0007 | ScyTek Laboratories | 7 ml | EUR 183 |
CD56; Clone 123C3 (Ready-To-Use) |
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| A00121-0025 | ScyTek Laboratories | 25 ml | EUR 449 |
) CD31; Clone C31.3 (Ready-To-Use) |
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| A00126-0002 | ScyTek Laboratories | 2 ml | EUR 82 |
CD31; Clone C31.3 (Ready-To-Use) |
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| A00126-0007 | ScyTek Laboratories | 7 ml | EUR 130 |
CD31; Clone C31.3 (Ready-To-Use) |
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| A00126-0025 | ScyTek Laboratories | 25 ml | EUR 290 |
) CD45; Clone 2B11 (Ready-To-Use) |
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| A00129-0002 | ScyTek Laboratories | 2 ml | EUR 120 |
CD45; Clone 2B11 (Ready-To-Use) |
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| A00129-0007 | ScyTek Laboratories | 7 ml | EUR 223 |
CD45; Clone 2B11 (Ready-To-Use) |
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| A00129-0025 | ScyTek Laboratories | 25 ml | EUR 572 |
) Tyrosinase; Clone T311 (Ready-To-Use) |
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| A00132-0002 | ScyTek Laboratories | 2 ml | EUR 90 |
Tyrosinase; Clone T311 (Ready-To-Use) |
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| A00132-0007 | ScyTek Laboratories | 7 ml | EUR 151 |
Tyrosinase; Clone T311 (Ready-To-Use) |
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| A00132-0025 | ScyTek Laboratories | 25 ml | EUR 353 |
) CDX2; Clone EP25 (Ready-To-Use) |
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| A00147-0002 | ScyTek Laboratories | 2 ml | EUR 149 |
CDX2; Clone EP25 (Ready-To-Use) |
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| A00147-0007 | ScyTek Laboratories | 7 ml | EUR 295 |
CDX2; Clone EP25 (Ready-To-Use) |
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| A00147-0025 | ScyTek Laboratories | 25 ml | EUR 786 |
) MSH6; Clone EP49 (Ready-To-Use) |
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| A00149-0002 | ScyTek Laboratories | 2 ml | EUR 104 |
MSH6; Clone EP49 (Ready-To-Use) |
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| A00149-0007 | ScyTek Laboratories | 7 ml | EUR 184 |
MSH6; Clone EP49 (Ready-To-Use) |
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| A00149-0025 | ScyTek Laboratories | 25 ml | EUR 453 |
 Ready-To-Use) Peroxide Block (Stable) Ready-To-Use |
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| HP1000-10 | Innovex | 10ml | EUR 125 |
Peroxide Block (Stable) Ready-To-Use |
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| HP1000-500 | Innovex | 500 ml | EUR 485 |
Peroxide Block (Stable) Ready-To-Use |
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| HP1000-60 | Innovex | 60 ml | EUR 241 |
 Ready-to-use Apoptosis Inducer Set |
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| K121-5 | Biovision | EUR 392 | |
 Green-DNA Dye, ready to use |
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| DT81414 | Bio Basic | 1.5ml, 1.5ml | EUR 128.3 |
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 Eco-Stain Plus, ready to use |
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| DT81418 | Bio Basic | 1ml | EUR 137 |
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 100-3000bp Marker, Ready-to-use |
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| GM347 | Bio Basic | 50loading, 50prep | EUR 90.02 |
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 Ready-to-Use Tyramide Amplification Buffer, 1X |
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| 9-22027 | Biotium |
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Description: Minimum order quantity: 1 unit of 25ML |
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 Ready-to-Use 1 KB DNA Ladder |
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| 31022 | Biotium | 1kit | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1kit |
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 Ready-to-Use 100 BP DNA Ladder |
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| 31032 | Biotium | 1kit | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1kit |
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) Bcl-2; Clone 124 (Ready-To-Use) |
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| A00004-0002 | ScyTek Laboratories | 2 ml | EUR 124 |
Bcl-2; Clone 124 (Ready-To-Use) |
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| A00004-0007 | ScyTek Laboratories | 7 ml | EUR 197 |
Bcl-2; Clone 124 (Ready-To-Use) |
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| A00004-0025 | ScyTek Laboratories | 25 ml | EUR 569 |
) p53; Clone DO-7 (Ready-To-Use) |
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| A00021-0002 | ScyTek Laboratories | 2 ml | EUR 168 |
p53; Clone DO-7 (Ready-To-Use) |
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| A00021-0007 | ScyTek Laboratories | 7 ml | EUR 287 |
p53; Clone DO-7 (Ready-To-Use) |
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| A00021-0025 | ScyTek Laboratories | 25 ml | EUR 882 |
) p53; Clone DO-1 (Ready-To-Use) |
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| A00027-0007 | ScyTek Laboratories | 7 ml | EUR 116 |
p53; Clone DO-1 (Ready-To-Use) |
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| A00027-0025 | ScyTek Laboratories | 25 ml | EUR 280 |
) Lambda, Light Chains; Polyclonal (Ready-To-Use) |
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| A00065-0002 | ScyTek Laboratories | 2 ml | EUR 79 |
Lambda, Light Chains; Polyclonal (Ready-To-Use) |
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| A00065-0007 | ScyTek Laboratories | 7 ml | EUR 108 |
Lambda, Light Chains; Polyclonal (Ready-To-Use) |
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| A00065-0025 | ScyTek Laboratories | 25 ml | EUR 252 |
) S-100; Clone 4C4.9 (Ready-To-Use) |
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| A00087-0002 | ScyTek Laboratories | 2 ml | EUR 79 |
S-100; Clone 4C4.9 (Ready-To-Use) |
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| A00087-0007 | ScyTek Laboratories | 7 ml | EUR 108 |
S-100; Clone 4C4.9 (Ready-To-Use) |
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| A00087-0025 | ScyTek Laboratories | 25 ml | EUR 252 |
S-100; Clone 4C4.9 (Ready-To-Use) |
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| A00087-0500 | ScyTek Laboratories | 500 ml | EUR 3227 |
S-100; Clone 4C4.9 (Ready-To-Use) |
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| A00087-1000 | ScyTek Laboratories | 1000 ml | EUR 4892 |
) Ki-67 Antigen; Polyclonal (Ready-To-Use) |
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| A00095-0002 | ScyTek Laboratories | 2 ml | EUR 116 |
Ki-67 Antigen; Polyclonal (Ready-To-Use) |
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| A00095-0007 | ScyTek Laboratories | 7 ml | EUR 181 |
Ki-67 Antigen; Polyclonal (Ready-To-Use) |
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| A00095-0025 | ScyTek Laboratories | 25 ml | EUR 509 |
; Clone Cocktail (Ready-To-Use)) Cytokeratin (Pan); Clone Cocktail (Ready-To-Use) |
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| A00098-0002 | ScyTek Laboratories | 2 ml | EUR 84 |
Cytokeratin (Pan); Clone Cocktail (Ready-To-Use) |
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| A00098-0007 | ScyTek Laboratories | 7 ml | EUR 116 |
Cytokeratin (Pan); Clone Cocktail (Ready-To-Use) |
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| A00098-0025 | ScyTek Laboratories | 25 ml | EUR 280 |
Cytokeratin (Pan); Clone Cocktail (Ready-To-Use) |
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| A00098-0500 | ScyTek Laboratories | 500 ml | EUR 3680 |
Cytokeratin (Pan); Clone Cocktail (Ready-To-Use) |
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| A00098-1000 | ScyTek Laboratories | 1000 ml | EUR 4892 |
) Estrogen Receptor; Clone 11D5 (Ready-To-Use) |
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| A00106-0002 | ScyTek Laboratories | 2 ml | EUR 162 |
Estrogen Receptor; Clone 11D5 (Ready-To-Use) |
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| A00106-0007 | ScyTek Laboratories | 7 ml | EUR 329 |
Estrogen Receptor; Clone 11D5 (Ready-To-Use) |
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| A00106-0025 | ScyTek Laboratories | 25 ml | EUR 887 |
) CA19-9; Clone 121SLE (Ready-To-Use) |
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| A00107-0002 | ScyTek Laboratories | 2 ml | EUR 120 |
CA19-9; Clone 121SLE (Ready-To-Use) |
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| A00107-0007 | ScyTek Laboratories | 7 ml | EUR 226 |
CA19-9; Clone 121SLE (Ready-To-Use) |
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| A00107-0025 | ScyTek Laboratories | 25 ml | EUR 580 |
) Insulin; Clone 2D11-H5 (Ready-To-Use) |
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| A00114-0002 | ScyTek Laboratories | 2 ml | EUR 69 |
Insulin; Clone 2D11-H5 (Ready-To-Use) |
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| A00114-0007 | ScyTek Laboratories | 7 ml | EUR 95 |
Insulin; Clone 2D11-H5 (Ready-To-Use) |
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| A00114-0025 | ScyTek Laboratories | 25 ml | EUR 188 |
; Clone 31A3 (Ready-To-Use)) UchL1 (PGP9.5); Clone 31A3 (Ready-To-Use) |
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| A00120-0002 | ScyTek Laboratories | 2 ml | EUR 69 |
UchL1 (PGP9.5); Clone 31A3 (Ready-To-Use) |
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| A00120-0007 | ScyTek Laboratories | 7 ml | EUR 95 |
UchL1 (PGP9.5); Clone 31A3 (Ready-To-Use) |
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| A00120-0025 | ScyTek Laboratories | 25 ml | EUR 188 |
) p21WAF1; Clone WA-1 (Ready-To-Use) |
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| A00125-0002 | ScyTek Laboratories | 2 ml | EUR 120 |
p21WAF1; Clone WA-1 (Ready-To-Use) |
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| A00125-0007 | ScyTek Laboratories | 7 ml | EUR 226 |
p21WAF1; Clone WA-1 (Ready-To-Use) |
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| A00125-0025 | ScyTek Laboratories | 25 ml | EUR 580 |
) Secretory Component; Clone SC05 (Ready-To-Use) |
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| A00127-0002 | ScyTek Laboratories | 2 ml | EUR 69 |
Secretory Component; Clone SC05 (Ready-To-Use) |
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| A00127-0007 | ScyTek Laboratories | 7 ml | EUR 95 |
Secretory Component; Clone SC05 (Ready-To-Use) |
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| A00127-0025 | ScyTek Laboratories | 25 ml | EUR 188 |
) Estrogen Receptor; Clone ERa078 (Ready-To-Use) |
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| A00128-0002 | ScyTek Laboratories | 2 ml | EUR 82 |
Estrogen Receptor; Clone ERa078 (Ready-To-Use) |
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| A00128-0007 | ScyTek Laboratories | 7 ml | EUR 130 |
Estrogen Receptor; Clone ERa078 (Ready-To-Use) |
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| A00128-0025 | ScyTek Laboratories | 25 ml | EUR 290 |
) CD45RB; Clone PD7/26 (Ready-To-Use) |
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| A00130-0002 | ScyTek Laboratories | 2 ml | EUR 77 |
CD45RB; Clone PD7/26 (Ready-To-Use) |
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| A00130-0007 | ScyTek Laboratories | 7 ml | EUR 116 |
CD45RB; Clone PD7/26 (Ready-To-Use) |
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| A00130-0025 | ScyTek Laboratories | 25 ml | EUR 248 |
) Cytokeratin 6; Clone EP67 (Ready-To-Use) |
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| A00140-0002 | ScyTek Laboratories | 2 ml | EUR 141 |
Cytokeratin 6; Clone EP67 (Ready-To-Use) |
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| A00140-0007 | ScyTek Laboratories | 7 ml | EUR 276 |
Cytokeratin 6; Clone EP67 (Ready-To-Use) |
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| A00140-0025 | ScyTek Laboratories | 25 ml | EUR 729 |
) Cytokeratin 20; Clone EP23 (Ready-To-Use) |
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| A00146-0002 | ScyTek Laboratories | 2 ml | EUR 151 |
Cytokeratin 20; Clone EP23 (Ready-To-Use) |
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| A00146-0007 | ScyTek Laboratories | 7 ml | EUR 303 |
Cytokeratin 20; Clone EP23 (Ready-To-Use) |
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| A00146-0025 | ScyTek Laboratories | 25 ml | EUR 809 |
) GFAP; Clone ASTRO/789 (Ready-To-Use) |
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| A00158-0002 | ScyTek Laboratories | 2 ml | EUR 87 |
GFAP; Clone ASTRO/789 (Ready-To-Use) |
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| A00158-0007 | ScyTek Laboratories | 7 ml | EUR 143 |
GFAP; Clone ASTRO/789 (Ready-To-Use) |
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| A00158-0025 | ScyTek Laboratories | 25 ml | EUR 329 |
) SOX10; Clone SOX10/991 (Ready-To-Use) |
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| A00161-0002 | ScyTek Laboratories | 2 ml | EUR 100 |
SOX10; Clone SOX10/991 (Ready-To-Use) |
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| A00161-0007 | ScyTek Laboratories | 7 ml | EUR 176 |
SOX10; Clone SOX10/991 (Ready-To-Use) |
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| A00161-0025 | ScyTek Laboratories | 25 ml | EUR 428 |
p53; Clone DO-1 (Ready-To-Use) |
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| A20027-0002 | ScyTek Laboratories | 2 ml | EUR 84 |
 Xpert Unstained Protein Marker, Ready-to-Use |
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| P8504-025 | GenDepot | 250ul | EUR 165 |
Xpert Unstained Protein Marker, Ready-to-Use |
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| P8504-050 | GenDepot | 2x250ul | EUR 219 |
 Mouse IgG Isotype control; Ready-To-Use |
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| NG903 | Innovex | 7 ml | EUR 241 |
 Mouse IgG1 Isotype control; Ready-To-Use |
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| NG904 | Innovex | 7 ml | EUR 269 |
 Mouse IgG2a Isotype control; Ready-To-Use |
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| NG905 | Innovex | 7 ml | EUR 334 |
 Mouse IgM Isotype control; Ready-To-Use |
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| NG906 | Innovex | 7 ml | EUR 311 |
 Mouse IgG2b Isotype control; Ready-To-Use |
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| NG907 | Innovex | 7 ml | EUR 334 |
 Mouse IgG3 Isotype control; Ready-To-Use |
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| NG908 | Innovex | 7 ml | EUR 334 |
 1Kb Plus DNA Ladder, Ready-to-use |
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| 9K-004-0001 | Bio Basic | 0.5ml | EUR 161.65 |
|
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 Eco-Red-DNA Dye, ready to use |
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| DT81415 | Bio Basic | 1ml | EUR 102.2 |
|
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 Eco-White-DNA Dye, ready to use |
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| DT81417 | Bio Basic | 1ml | EUR 102.2 |
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 100-1200bp DNA Marker, Ready-to-use |
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| GM333 | Bio Basic | 50loading, 50prep | EUR 67.4 |
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Recently, a two-column steady implementation of frontal chromatography, known as Flow2, was developed (Vogg et al., J. Chrom. A, 1619, 460943, 2020). Despite having the ability of assuaging the purity-yield tradeoff typical of batch operations, the enhance in the quantity of course of parameters complicates its optimum design, with the threat of not exploiting its full potential. In this work, we developed an advert hoc design process appropriate for the optimization of each batch frontal chromatography and Flow2 in phrases of purity, yield and productiveness. This process supplied comparable outcomes as a multi-objective optimization primarily based on genetic algorithm however with decrease computational effort.