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immunoblot

Western Blotting (also referred to as immunoblotting) is a technique used for analysis of individual proteins within a protein mix (e.g. a cell lysate). In Western blotting (immunoblotting) the protein mix is applied to a gel electrophoresis at a carrier matrix (SDS-PAGE, native PAGE, isoelectric focusing, 2D gel electrophoresis, etc.) to sort the proteins by …

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BlazeTaq One-Step

BlazeTaq One-Step SYBR Green RT-qPCR Kit (100 qPCR Responses ) A. Amplification curves of GAPDH and Actin respectively in HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg; B. Amplification curves of GAPDH and Actin respectively in HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg; C. Melting curves …

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qRT-PCR SuperMix Kit

RT-qPCR Kits for RT-qPCR, or quantitative reverse transcriptase PCR or Real Time RT-PCR, Empower quantitative PCR Straight from RNA samples. These ready-to-use kits include the necessary components for carrying both cDNA synthesis and qPCR. Kit components typically consist of reverse transcriptase, polymerase, buffer, fluorescent probes, and dNTPs. Added reagents such as RNase inhibitors and enhancers …

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cDNA-Random Hexamer

One-step RT-PCR reactions consistently use a gene-specific primer for first-strand cDNA synthesis, while two-step RT-PCR reactions permit for additional priming choices. “When the mRNA includes a poly-A 3′ tail, then a oligo-dT primer may be used to prime each of mRNAs simultaneously. SSPs give the best specificity and also have been proven to be the …

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rt pcr lab

What is RT-PCR?

For RT-PCR the RNA molecules are transformed into their complementary DNA (cDNA) sequences by reverse transcriptase. (with Mulv) The next step is the amplification of the newly synthesized cDNA by standard TAQ polymerase PCR procedures. This approach to examine gene expression is universally known as RT-PCR, because of the role of reverse transcriptase (RT) in …

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