immunoblot

Western Blotting (also referred to as immunoblotting) is a technique used for analysis of individual proteins within a protein mix (e.g. a cell lysate). In Western blotting (immunoblotting) the protein mix is applied to a gel electrophoresis at a carrier matrix (SDS-PAGE, native PAGE, isoelectric focusing, 2D gel electrophoresis, etc.) to sort the proteins by dimension , charge, or other…

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GPR160 Lentiviral Vector Human pLenti GIII

Product Records Insert Size 4206 bp Are designing primers using a Kozak sequence demanded or is ATG enough? Why is my lentiviral infection of lower efficacy than transduction? Product Application How should I store my lentivirus? Guarantee abm guarantees that the proper ORF construct is provided along with the mRNA expression is displayed upon successful transduction. If that is not…

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BlazeTaq One-Step

BlazeTaq One-Step SYBR Green RT-qPCR Kit (100 qPCR Responses ) A. Amplification curves of GAPDH and Actin respectively in HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg; B. Amplification curves of GAPDH and Actin respectively in HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg; C. Melting curves of the amplified fragments of…

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qRT-PCR SuperMix Kit

RT-qPCR Kits for RT-qPCR, or quantitative reverse transcriptase PCR or Real Time RT-PCR, Empower quantitative PCR Straight from RNA samples. These ready-to-use kits include the necessary components for carrying both cDNA synthesis and qPCR. Kit components typically consist of reverse transcriptase, polymerase, buffer, fluorescent probes, and dNTPs. Added reagents such as RNase inhibitors and enhancers may also be contained. For…

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cDNA-Random Hexamer

One-step RT-PCR reactions consistently use a gene-specific primer for first-strand cDNA synthesis, while two-step RT-PCR reactions permit for additional priming choices. “When the mRNA includes a poly-A 3′ tail, then a oligo-dT primer may be used to prime each of mRNAs simultaneously. SSPs give the best specificity and also have been proven to be the most persistent of the primer…

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rt pcr lab

What is RT-PCR?

For RT-PCR the RNA molecules are transformed into their complementary DNA (cDNA) sequences by reverse transcriptase. (with Mulv) The next step is the amplification of the newly synthesized cDNA by standard TAQ polymerase PCR procedures. This approach to examine gene expression is universally known as RT-PCR, because of the role of reverse transcriptase (RT) in the synthesis of first-strand cDNA.…

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