BlazeTaq One-Step SYBR Green RT-qPCR Kit (100 qPCR Responses )
A. Amplification curves of GAPDH and Actin respectively in HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg; B. Amplification curves of GAPDH and Actin respectively in HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg; C. Melting curves of the amplified fragments of GAPDH (reddish ) and Actin (blue).
Identification of the level of Actin and GAPDH extract from HeLa cells.
• One-step reactions to measure the relative Quantity of RNA
User Guide
CatNumber: QP070-GC
Comparison of the functionality of BlazeTaq™ RT-qPCR kit with rival ´s merchandise.
BlazeTaq™ One-Step SYBR® Green RT-qPCR kit employs reverse transcriptase to convert RNA into cDNA, and also an antibody-modified Taq DNA polymerase to steer clear of polymerase action before thermal cycling. The antibody dissociates and activity of the Taq polymerase is revived upon heating for 3 minutes. In addition, the buffer system enables amplification efficiency and specificity, in addition to enhancing sensitivity of real-time PCR reactions .
• High amplification performance over broad GC-content Variety
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• High specificity, together with a minimal degree of primer-dimer and non invasive product formation
A. Amplification curves of GAPDH in HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg using BlazeTaq™ (reddish ) and Competitor P´s kit (blue); B. Melting curves of amplified fragments of GAPDH with BlazeTaq™ (reddish ) along with Competitor P kit (blue). Amplification is shown by the summit from rival.
Comparison of the operation of BlazeTaq™ RT-qPCR kit with rival ´s merchandise.
Performance
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Cost: 187 $
A. Amplification curves of GAPDH in HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg with BlazeTaq™ (reddish ) and Competitor T´s kit (blue); B. Amplification curves of Actin from HeLa cells by RNA infusion ranging from 100 ng to 0.1 pg using BlazeTaq™ (reddish ) along with Competitor T´s kit (blue).
Provider: GeneCopoeia